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Bioinformatics analysis of Nanopore sequencing data for SARS-CoV-2

By March 29, 2020News
Man in Blue Collared Top Using Imac Indoors Free Stock Photo

Man in Blue Collared Top Using Imac Indoors Free Stock Photo

The study of the COVID-19 pandemic often involves sequencing the SARS-CoV-2 virus using Illumina, Nanopore, IonTorrent and Sanger technologies alone or in combination. Depending on the sampling and library preparation method used, this can involve metatranscriptomic data of varying viral content.

In this webinar tutorial, the following topics will be discussed:

  • Best practices for bioinformatic analyses using the versatile QIAGEN CLC Genomics Workbench
  • QC, adapter trimming and strategies for host read depletion and viral content enrichment
  • Simple pipelines for assembly and strain identification
  • A demonstration of read mapping, variant calling and consensus sequence extraction, which are necessary steps for obtaining high-quality genomic sequences for phylogenetic analysis and publication

 

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